| APOLD1

Apolipoprotein L domain containing 1 is a protein that is encoded by the APOLD1 gene. It is located on Chromosome 12.APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.33	1.76	1.16	0.7	0.46	0.33	0.24	0.1
Corrected OD	2.23	1.66	1.06	0.6	0.36	0.23	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human APOLD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	85-101	96
Cell Culture Supernates(n=8)	92-105	103

 

| Linearity

Samples were spiked with high concentrations of Human APOLD1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	91	94
1 : 4	Range (%)	89-103	88-108
	Average (%)	94	98